Search results for "molecular investigation"
showing 6 items of 6 documents
DNA analysis as tool for identification of bacteria in archaeological waterlogged wood
2014
Abstract In this work molecular techniques were applied in order to integrete the results obtained by Optical (OM) and Scanning Electron (SEM) Microscopy, to understanding and assessing the changes in the anatomical structure of archaeological waterlogged wood (Pinus sp.) induced by bacteria colonization. Observation of wooden thin sections revealed by OM showed the presence of black and dark -brown areas (must probably due to sulphur compounds) and mineral concretions. The SEM micrographs revealed a specific cell wall alteration attributable to bacterial activity and abundant pyrite framboids (as single structure or clustered). The presence of sulfur compounds in archaeological waterlogged…
Contribution to the identification of Dianthus rupicola (Caryophyllaceae) subspecies using morphological and molecular approaches
2017
The systematic relationships between the five different subspecies actually recognized within Dianthus rupicola (subsp. aeolicus , subsp. bocchorianus , subsp. hermaensis, subsp. lopadusanus and subsp. rupicola ), were assessed by means of morphometric and molecular analysis. Our results highlighted the molecular differences between the five subspecies of D. rupicola and a morphological differentiation of four groups being D. rupicola subsp. aeolicus and D. rupicola subsp. rupicola partly overlapping. At the present state of knowledge, a subspecific status seems the most appropriate for all the recognized subspecies of D. rupicola . The names D. aeolicus , D. bisignanii var. virescens , D. …
Monitoring Biological Damage on Paper-based Documents in the Historical Archive of the Palermo Astronomical Observatory
2017
Libraries and archives house a wide variety of documents made of materials of vegetal and animal origin: unbound papers and books, manuscripts and printed books, photographs (negative and positive), prints, maps, available to the public for reading needs, study and information. These materials are often subject to fluctuations in environmental and micro-environmental parameters. For this reason, it is essential to implement constant monitoring and control of environmental conditions and potential deteriogens in order to slow down deterioration processes. The monitoring of the microbial degradation of paper documents in the Historical Archives of Palermo Astronomical Observatory has revealed…
Bacteria consortia and deterioration of archaeological waterlogged wood: identification by molecular and microscopy techniques
2014
In this study molecular tools are applied to reveal and identify bacterial colonization in waterlogged wood to assessing the changes induced in anatomical structure, previously observed by Optical and Scanning Electron Microscopy (1). The results obtained by observation of wooden thin sections (OM), shown the presence of black and dark-brown areas and mineral concretions. The SEM analysis revealed a specific cell walls alteration, attributable to bacterial activity, other than abundant pyrite framboids (FeS2). The presence of sulfur compounds in archaeological waterlogged wood can indicate both long-term burial in anoxic environment and colonization by sulfate-reducing bacteria. Molecular m…
Serological and molecular identification of Legionella spp. isolated from water and surrounding air samples in Italian healthcare facilities
2016
Abstract Background Legionella is an intracellular microorganism living in natural and artificial aquatic environments. Although its transmission to humans is linked to the inhalation of contaminated aerosols, there is no validated air sampling method for the control and prevention of the disease. The aim of the present study was to provide more information on the distribution of Legionella spp. in indoor environments and to determine whether the same Legionella strains are isolated from air and water samples. Methods Ten healthcare facilities located in seven regions of Italy were enrolled. The serological typing of Legionella spp. from water samples and the surrounding air by active and p…
Plant products as biocides for conservation of cultural asset sustainable for human and environmental health
2022
In the last decades plants products are increasingly used also in defining innovative procedures for sustainable conservation of cultural heritage. Particularly, essential oils or hydro-alcoholic extracts have been fruitful applied to contrast microbial colonization on organic and inorganic artworks or insect infestation (Anobidae) having regard to the repellent action.
 In this paper, extracts from Asteraceae and Lamiaceae plant families are utilized to counteract widespread microbial colonization (bacteria, cyanobacteria, fungi) due to their antimicrobial activity. In order to define the adequate concentration correlating it to microbial species detected, the antimicrobial activity o…